Biosoft PrimerPlex v2.11.21103 PCR或測序引物以及雜交探針的設計評估軟體 英文破解版
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Biosoft PrimerPlex v2.11.21103 PCR或測序引物以及雜交探針的設計評估軟體 英文破解版
^^^^^^^^^^^^^^^^^^^^^^^^ 2010年9月最新版!
For WIN 9x/WIN ME/WIN NT/WIN 2000/WIN XP/WIN 2003/WIN VISTA
原版光碟!!!絕對超值!!
2010.09.05 全新〞光榮 〞上市 軟體急先鋒(http://83.to/twvcd)
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Biosoft PrimerPlex v2.11.21103 PCR或測序引物以及雜交探針的設計評估軟體 英文破解版
破解說明：安裝完成後，請將光碟 \crack 目錄下的patcher，複製到主程式的
安裝目錄內中，並覆蓋!
"(install)\http\Dwww.premierbiosoft.com\P80\DMIUpdater\DMP2\DM210"
from cmd prompt run java -jar patcher.jar
官方網站：http://www.premierbiosoft.com/primerdesign/index.html
由加拿大的Premier公司開發的專業用於PCR或測序引物以及雜交探針的設計，評估的軟體，
其主要介面是分為序列編輯視窗（Genetank），引物設計視窗（Primer Design），瑂切分
析視窗（Restriction Sites）和紋基分析視窗（Motif）。
Primer Premier's search algorithm finds optimal PCR, multiplex, SNP genotyping
and degenerate primers with the most accurate melting temperature using the
nearest neighbor thermodynamic algorithm. Primers are screened for secondary
structures, dimers, hairpins, homologies and physical properties before reporting
the best ones for your sequence, in ranked order. Equipped with a handy calculator,
you can easily manipulate sequences and analyze the results of your primer design.
Load the gene of interest from NCBI, select a search range, sit back and let
Primer Premier pick the best possible primers for you.
Primer Design for SNP Genotyping Assays
With Primer Premier, you can load sequences from dbSNP and have the primers designed
flanking the SNP selected. Hundreds of unpublished SNPs can also be loaded by
specifying them as variation features in standard GenBank/dbSNP files. After specifying
the SNPs, primers can be designed to amplify them for detection using a probe-based
chemistry.
Multiplex Primer Design
For a multiplex experiment, Primer Premier enables you to design multiplex primers by
launching a primer search in batch mode and then checking the cross reactivity of the
primers designed. Primer Premier checks for all the possible reactivity and displays the
most stable structure formed by each oligo. This functionality reduces false priming and
ensures a strong signal strength.
Automatic Homology & Template Structure Avoidance
Primer Premier automatically interprets the BLAST search results and avoids those regions
to design primers that have significant cross homologies with the database. These homologous
regions are highlighted in the sequence view and are avoided during primer search.
Primer extension may be hindered due to the presence of template structures at extension
temperature. To avoid all such regions, where the template may fold upon itself, the program
utilizes a proprietary algorithm to check for possible secondary structures within the
template at a folding temperature you specify. The regions involved in the formation of a
secondary structure are underlined in the sequence view and are avoided while designing
primers.
Avoiding homologous regions makes the oligos highly specific and avoiding template structures
improves the efficiency of the designed primers.
軟體急先鋒 強力推薦！！！一定讓你值回票價，保證錯不了。
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軟體急先鋒(http://83.to/twvcd) 目錄編輯
最後，有句老話還是要再強調一次！軟體急先鋒製作的目錄，僅僅只是幫助您試用/選購各
種應用軟體之用，如果您覺得這些試用的軟體真的對您工作上真正有幫助，敬請務
必要買原版軟體，以支援作者或出版公司能再出版最好的軟體!!! 為了您將來的後
續服務，更應該要購買原版的軟體！謝謝!!!
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